Published in Scientific Papers. Series E. Land Reclamation, Earth Observation & Surveying, Environmental Engineering, Vol. XIII
Written by Iuliana Diana BĂRBULESCU, Raluca Ștefania RĂDOI-ENCEA, Corina DUMITRACHE, Mihaela Violeta GHICA, Mihai FRÎNCU, Mihaela BEGEA, Valerica TUDOR, Elena-Mirela BOROIU, Răzvan Ionuț TEODORESCU
The present work describes the optimization of the composition of liquid culture medium in sub-merged fermentation in batch system by varying 7 factors, according to a Hadamard matrix. The work aimed to determine the optimal values of the varied factors (sugar content, yeast extract, KCl, medium volume (Vw/Vt), inoculation ratio, MgSO4 × 7H2O, vitamins) that conducted to a maximum value of the system response, namely the yeast biomass cell weight, (6.61 g) a parameter relevant from technical and economic point of view. The temperature, stirring rate and air flow rate for the Hadamard matrix were maintained constant for each process variant. Applying the process, an active yeast biomass with a protein content of 36.45 g% was obtained. The active yeast biomass of S. cerevisiae PFE-II-3 was used in a further study to obtain wines at the Pietroasa Viticulture and Winemaking Research and Development Station.
[Read full article] [Citation]
